In recent years, proteome research has become more and more involved in the clarification of various disease mechanisms. Proteins can reflect the current condition of a disease and guide treatment. Through the comparative analysis of the proteome, we can find specific proteins that have the potential to become molecular targets of new drug designs and provide molecular markers for the early diagnosis of diseases. Due to the development of mass spectrometry technology, currently, our understanding of the proteome in organisms is more comprehensive and accurate. Compared to other proteomics techniques, 4D label-free proteomics has faster speed, higher sensitivity, and stronger stability. It can detect proteins in various matrices, including micro samples such as single cells, serum, plasma, cerebrospinal fluid, and urine.
Example
Pathway analysis reveals characteristics of endometrioid carcinoma and HGSC
Proteins significantly elevated in endometrioid carcinoma (A) or HGSC (B) samples were analyzed in Metascape, and the top 20 significant overrepresented pathways are shown. (C) Heatmap comparing similarities and differences between highly significant process and pathways. (D) Heatmap showing normalized (z-scored) expression of core proteins matching to estrogen response late or antiviral mechanism by IFN-stimulated genes. Proteins significantly elevated in endometrioid carcinoma (A) or HGSC (B) samples were analyzed in Metascape, and the top 20 significant overrepresented pathways are shown. (C) Heatmap comparing similarities and differences between highly significant process and pathways. (D) Heatmap showing normalized (z-scored) expression of core proteins matching to estrogen response late or antiviral mechanism by IFN-stimulated genes.
References
Shanghai Outdo Biotech Co.,LTD http://www.superchip.com.cn/biology/index.html
Dieters-Castator DZ, Rambau PF, Kelemen LE, et al. Proteomics-Derived Biomarker Panel Improves Diagnostic Precision to Classify Endometrioid and High-grade Serous Ovarian Carcinoma. Clin Cancer Res. 2019;25(14):4309-4319. doi:10.1158/1078-0432.CCR-18-3818
