Cell surface markers and several phosphorylation events can be simultaneously examined in each cell, allowing for the resolution of complex biochemical signaling networks in heterogeneous cell populations. The technique has been used in a wide range of biological contexts, including high-throughput, high-content drug discovery, characterization of signaling pathways in immune responses to antigenic stimulation and microbial challenge, and alteration of signaling networks in cancer and autoimmune diseases.
Method
Phospho-specific flow Flow cytometry, or phospho flow, is used for the measurement of the phosphorylation state of intracellular proteins at the single cell level.
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Example
Blood phospho-tau in alzheimer's disease
Multicolor analysis of human whole blood (A), or human PBMCs (B), stimulated IL-4/IL-6 treated and stained with anti-human CD3, anti-human CD4, anti-Stat 6 (pY641), and anti-Stat 3 (pY705).
Reference
Karikari, T.K., Ashton, N.J., Brinkmalm, G. et al. Blood phospho-tau in Alzheimer disease: analysis, interpretation, and clinical utility. Nat Rev Neurol 18, 400–418 (2022). https://doi.org/10.1038/s41582-022-00665-2
