Tumor Immune Microenvironment Phenotyping

A deeper understanding of the tumor microenvironment is necessary for improved patient prognosis and therapeutic management, and this involves a thorough characterization of the immune populations that infiltrate the tumor.

Method

Flow cytometry

Gating strategy for flow cytometry analysis of the tumor microenvironment

The figure depicts the gating strategies for identifying tumor cells, OT-I T cells, NK cells, monocytes and neutrophils present in the bone marrow of tumor bearing mice.

Reference

Thibaut, R., Bost, P., Milo, I. et al. Bystander IFN-γ activity promotes widespread and sustained cytokine signaling altering the tumor microenvironment. Nat Cancer 1, 302–314 (2020). https://doi.org/10.1038/s43018-020-0038-2