In Vitro Toxicology Studies

Computer Modeled Toxicity Prediction

• Derek is a toxicity prediction software based on an "expert knowledge base"

• Sarah is a toxicity prediction software built on a "statistical model“

Genotoxicity Assessment

• Ames

• Mini-Ames (6-well plate)

• Chromosomal aberrations in vitro (chamber slides)

• In vitro binuclear micronucleus (chamber slide/96-well plate)

• In vitro photogenotoxicity test

• In vitro multi-biomarker genotoxicity (γ-H2AX, p53, H3, Cleaved-PARP)

Reproductive and Developmental Toxicity Assessment

• Whole embryo culture in vitro

• Developmental toxicity screening of zebrafish embryos

Cardiotoxicity Evaluation

• Cardiac Ion Channel Screening Assay (IC50)

• hERG potassium channel (HEK293 cells transfected with the hERG gene)

• Nav1.5 sodium channel (HEK293 cells transfected with Nav1.5 gene)

• Cav1.2 calcium channel (CHO cells transfected with Cav1.2 gene

• xCelligence Cardio Analysis System in Real Time

• Rat primary cardiomyocytes

• Human induced pluripotent stem cell differentiated cardiomyocytes (hiPSC-CM)

Action Potential Test on Isolated Myocardial Tissue

• Guinea pig papillary muscle action potential

• Rabbit Purkinje fiber action potential

• In vitro evaluation of carcinogenicity

• Based on the Bhas42-transformed cell system, the cytotoxicity test was used to determine the carcinogenic effect of the compound and the concentration of the growth-promoting test.

• Based on the Bhas42 transformed cell system, the genes related to the initiation stage and the growth-promoting stage of carcinogenesis were screened by the gene chip method.

Immunotoxicity

• Chemokines and cytokines changes in immune cell count.

• T-cell dependent antibody response (TDAR)

• Immunophenotyping